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human stat2 sirna catalogue  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology human stat2 sirna catalogue
    Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and <t>STAT2-null</t> U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.
    Human Stat2 Sirna Catalogue, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+stat2+sirna+catalogue/Stat2+siRNA/pmc04215738-100-22-12
    Average 88 stars, based on 12 article reviews
    human stat2 sirna catalogue - by Bioz Stars, 2026-09
    88/100 stars

    Images

    1) Product Images from "STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2"

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    Journal: Journal of molecular biochemistry

    doi:

    Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and STAT2-null U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.
    Figure Legend Snippet: Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and STAT2-null U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.

    Techniques Used: Expressing, Incubation, Fluorescence, Flow Cytometry, Control

    Effect of STAT3 inhibition on U3A and U6A survival. (A) AG490 treatment x 24 hr; apoptosis. Effect of AG490 treatment on STAT1 or 2 and time they were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. (B) Effect of 13410 and 13410a in STAT1- and STAT2-null cells. U3A and U6A cells were treated with STAT3 inhibitors 13410 and 13410a and control oligonucleotides 13778 and 13778a for 48 hr. Cells were harvested and stained with FITC-annexin V/PI to assess apoptosis by flow cytometry. Apoptosis experiments were conducted three times. Data from a representative experiment are given.
    Figure Legend Snippet: Effect of STAT3 inhibition on U3A and U6A survival. (A) AG490 treatment x 24 hr; apoptosis. Effect of AG490 treatment on STAT1 or 2 and time they were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. (B) Effect of 13410 and 13410a in STAT1- and STAT2-null cells. U3A and U6A cells were treated with STAT3 inhibitors 13410 and 13410a and control oligonucleotides 13778 and 13778a for 48 hr. Cells were harvested and stained with FITC-annexin V/PI to assess apoptosis by flow cytometry. Apoptosis experiments were conducted three times. Data from a representative experiment are given.

    Techniques Used: Inhibition, Staining, Flow Cytometry, Control

    Effect of STAT1 and STAT2 gene silencing with siRNA in STAT3-dependent cell lines. DU-145 and PANC-1 cells were treated with 500 nM pooled siRNA for 72 hr, at which time cells were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. More than 95% of the cells were observed to be viable following siRNA transfection. Transfection efficiencies ranged from 95 to 98% (not shown).
    Figure Legend Snippet: Effect of STAT1 and STAT2 gene silencing with siRNA in STAT3-dependent cell lines. DU-145 and PANC-1 cells were treated with 500 nM pooled siRNA for 72 hr, at which time cells were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. More than 95% of the cells were observed to be viable following siRNA transfection. Transfection efficiencies ranged from 95 to 98% (not shown).

    Techniques Used: Staining, Flow Cytometry, Transfection

    Effect of siRNA-mediation inhibition of STATs 1, 2, and 3 on cell survival. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. Data from a representative experiment are given.
    Figure Legend Snippet: Effect of siRNA-mediation inhibition of STATs 1, 2, and 3 on cell survival. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. Data from a representative experiment are given.

    Techniques Used: Inhibition, Transfection, Staining, Flow Cytometry, Control

    Lack of synergy between STAT1 or STAT2 and STAT3 siRNAs. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were co-transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. % apoptotic data were normalized to 100% transfection efficiency. P value was determined by repeated ANOVA.
    Figure Legend Snippet: Lack of synergy between STAT1 or STAT2 and STAT3 siRNAs. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were co-transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. % apoptotic data were normalized to 100% transfection efficiency. P value was determined by repeated ANOVA.

    Techniques Used: Transfection, Staining, Flow Cytometry, Control



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    human stat2 sirna catalogue  (Santa Cruz Biotechnology)
    88
    Santa Cruz Biotechnology human stat2 sirna catalogue
    Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and <t>STAT2-null</t> U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.
    Human Stat2 Sirna Catalogue, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+stat2+sirna+catalogue/Stat2+siRNA/pmc04215738-100-22-12
    Average 88 stars, based on 1 article reviews
    human stat2 sirna catalogue - by Bioz Stars, 2026-09
    88/100 stars
    		  Buy from Supplier

    Image Search Results


    Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and STAT2-null U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.

    Journal: Journal of molecular biochemistry

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    doi:

    Figure Lengend Snippet: Phospho-STAT3 expression in 2fTGH, U3A and U6A cells. Expression of activated (phospho) STAT3 by fibrosarcoma cell lines. Parental 2fTGH, STAT1-null U3A, and STAT2-null U6A cells were fixed, permeabilized, blocked appropriately and then incubated with PE-anti-phospho-STAT3 (Y-705) Ab. Fluorescence was quantified by flow cytometry. Green = control Ab (anti-P-STAT5 Ab); red = anti-P-STAT3 Ab.

    Article Snippet: Pooled siRNA oligonucleotides to silence STAT1, STAT2, and STAT3 were obtained from Santa Cruz Biotechnology; specifically the human STAT1 siRNA catalogue #sc-44123, human STAT2 siRNA catalogue #sc-29492, and human STAT3 siRNA catalogue #sc-29493 were used.

    Techniques: Expressing, Incubation, Fluorescence, Flow Cytometry, Control

    Effect of STAT3 inhibition on U3A and U6A survival. (A) AG490 treatment x 24 hr; apoptosis. Effect of AG490 treatment on STAT1 or 2 and time they were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. (B) Effect of 13410 and 13410a in STAT1- and STAT2-null cells. U3A and U6A cells were treated with STAT3 inhibitors 13410 and 13410a and control oligonucleotides 13778 and 13778a for 48 hr. Cells were harvested and stained with FITC-annexin V/PI to assess apoptosis by flow cytometry. Apoptosis experiments were conducted three times. Data from a representative experiment are given.

    Journal: Journal of molecular biochemistry

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    doi:

    Figure Lengend Snippet: Effect of STAT3 inhibition on U3A and U6A survival. (A) AG490 treatment x 24 hr; apoptosis. Effect of AG490 treatment on STAT1 or 2 and time they were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. (B) Effect of 13410 and 13410a in STAT1- and STAT2-null cells. U3A and U6A cells were treated with STAT3 inhibitors 13410 and 13410a and control oligonucleotides 13778 and 13778a for 48 hr. Cells were harvested and stained with FITC-annexin V/PI to assess apoptosis by flow cytometry. Apoptosis experiments were conducted three times. Data from a representative experiment are given.

    Article Snippet: Pooled siRNA oligonucleotides to silence STAT1, STAT2, and STAT3 were obtained from Santa Cruz Biotechnology; specifically the human STAT1 siRNA catalogue #sc-44123, human STAT2 siRNA catalogue #sc-29492, and human STAT3 siRNA catalogue #sc-29493 were used.

    Techniques: Inhibition, Staining, Flow Cytometry, Control

    Effect of STAT1 and STAT2 gene silencing with siRNA in STAT3-dependent cell lines. DU-145 and PANC-1 cells were treated with 500 nM pooled siRNA for 72 hr, at which time cells were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. More than 95% of the cells were observed to be viable following siRNA transfection. Transfection efficiencies ranged from 95 to 98% (not shown).

    Journal: Journal of molecular biochemistry

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    doi:

    Figure Lengend Snippet: Effect of STAT1 and STAT2 gene silencing with siRNA in STAT3-dependent cell lines. DU-145 and PANC-1 cells were treated with 500 nM pooled siRNA for 72 hr, at which time cells were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. More than 95% of the cells were observed to be viable following siRNA transfection. Transfection efficiencies ranged from 95 to 98% (not shown).

    Article Snippet: Pooled siRNA oligonucleotides to silence STAT1, STAT2, and STAT3 were obtained from Santa Cruz Biotechnology; specifically the human STAT1 siRNA catalogue #sc-44123, human STAT2 siRNA catalogue #sc-29492, and human STAT3 siRNA catalogue #sc-29493 were used.

    Techniques: Staining, Flow Cytometry, Transfection

    Effect of siRNA-mediation inhibition of STATs 1, 2, and 3 on cell survival. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. Data from a representative experiment are given.

    Journal: Journal of molecular biochemistry

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    doi:

    Figure Lengend Snippet: Effect of siRNA-mediation inhibition of STATs 1, 2, and 3 on cell survival. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. Data from a representative experiment are given.

    Article Snippet: Pooled siRNA oligonucleotides to silence STAT1, STAT2, and STAT3 were obtained from Santa Cruz Biotechnology; specifically the human STAT1 siRNA catalogue #sc-44123, human STAT2 siRNA catalogue #sc-29492, and human STAT3 siRNA catalogue #sc-29493 were used.

    Techniques: Inhibition, Transfection, Staining, Flow Cytometry, Control

    Lack of synergy between STAT1 or STAT2 and STAT3 siRNAs. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were co-transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. % apoptotic data were normalized to 100% transfection efficiency. P value was determined by repeated ANOVA.

    Journal: Journal of molecular biochemistry

    Article Title: STAT3 Inhibition Induces Apoptosis in Cancer Cells Independent of STAT1 or STAT2

    doi:

    Figure Lengend Snippet: Lack of synergy between STAT1 or STAT2 and STAT3 siRNAs. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were co-transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. % apoptotic data were normalized to 100% transfection efficiency. P value was determined by repeated ANOVA.

    Article Snippet: Pooled siRNA oligonucleotides to silence STAT1, STAT2, and STAT3 were obtained from Santa Cruz Biotechnology; specifically the human STAT1 siRNA catalogue #sc-44123, human STAT2 siRNA catalogue #sc-29492, and human STAT3 siRNA catalogue #sc-29493 were used.

    Techniques: Transfection, Staining, Flow Cytometry, Control